Lyophilized solid. Lyophilized. Recombinant, Pyrococcus furiosus pyroglutamyl aminopeptidase expressed in E. coli. A proteolytic enzyme that cleaves pyroglutamate from the N-terminus of polypeptide chains. Useful in protein sequencing to unblock proteins and peptides with N-terminal pyroglutaryl residues. Specific activity: ≥10 units/mg protein. One unit is defined as the amount of enzyme that will hydrolyze 1 µmol of Pyr-pNA per min at 37°C, pH 7.0. Purity: ≥90% by SDS-PAGE. Contaminants: proteases: none detected. Reconstitute in 50 mM sodium phosphate buffer, 10 mM DTT, 1 mM EDTA, pH 7.0. pH optimum 6.0 - 9.0. EC 3.4.19.3. Note: 1 mU = 1 milliunit

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