rH-Ras, WT Liquid. In 45 mM NaCl, 20 mM Tris-HCl, 5 mM MgCl2, 1 mM DTT, 50% glycerol, pH 8.0. AVOID FREEZE/THAW CYCLES. Recombinant, human wild-type Ras expressed in E. coli. Suitable as a substrate for farnesyltransferase. Activity: GDP binding: ≥1:4 stoichiometry of GDP:H-Ras. Purity: ≥90% by SDS-PAGE. Ref.: Marshall, M.S. 1995. FASEB J. 9, 1311. James, G.L., et al. 1993. Science 260, 1937. Reiss, Y., et al. 1990. Cell 62, 81. |
