Liquid. In 20 mM HEPES, 5 mM MgCl2, 40% glycerol, 0.05% NaN3, pH 8.0. AVOID FREEZE/THAW CYCLES. Recombinant, human Rho fused to GST and expressed in E. coli. A small GTPase of the Ras superfamily. Regulates stress fiber formation in response to growth factor stimulation. Useful as a positive control in Western analysis and as an affinity precipitation reagent using immobilized glutathione resin. Activity: GTPgS binding ≥400 mmol/mol. Purity: ≥90% by SDS-PAGE. Ref.: Ridley, A. 1996. Curr. Biol. 6, 1256. Ridley, A. and Hall, A. 1992. Cell 70, 389. |
