Liquid. In 50 mM KCl, 20 mM HEPES, 8 mM DTT, 50% glycerol. Recombinant, human ribonuclease inhibitor expressed in E. coli. Non-competitive inhibitor that inactivates RNase by non-covalent binding. Has been used to improve cDNA synthesis and in vitro RNA synthesis, increase yields of polysomes, and aid in the preparation of RNase-free antibodies. Inhibits RNases A, B, and C. Does not inhibit RNase T1 and S1 nuclease from Aspergillus. Activity: ≥20,000 units/ml. One unit is defined as the amount of material that will inhibit 50% of the activity of 5 ng of RNase A. Contaminants: Endonuclease and RNase: none detected. Ref.: Saxena, S.K., et al. 1992. J. Biol. Chem. 267, 21982. Lee, F.S., et al. 1989. Biochemistry 28, 225. |
