RNA Polymerase II, Carboxy-Terminal Domain

Liquid. In 100 mM KCl, 20 mM Tris-HCl, 1 mM DTT, 200 µM EDTA, 20% glycerol, pH 7.9. AVOID FREEZE/THAW CYCLES. Recombinant carboxy-terminal repeat domain (CTD) of the largest subunit of RNA polymerase II expressed in E. coli. Contains tandem repeats of a heptapeptide sequence Tyr-Ser-Pro-Thr-Ser-Pro-Ser, which is highly conserved among eukaryotic organisms. The RNA pol II-CTD is essential for transcription, for organization of transcription foci within the nucleus, and for pre-mRNA processing, and is targeted by several kinases and phosphatases. Activity: 1 unit/ng protein. Biological activity: 20 units are sufficient for reconstituted transcription assays; 100 units are sufficient for protein-protein interaction assays.. Purity: ≥95% by SDS-PAGE. Contaminants: DNase, RNase, and protease activity: none detected. Note: 1 KU = 1000 units

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