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| | PhosphoDetect™ Anti-SAPK/JNK (pThr183, Tyr185) Rabbit pAb |
|  | Cat. No. 559309 |
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| Host: Rabbit | | Isotype: IgG | | Immunogen: a synthetic phosphopeptide corresponding to amino acids surrounding the Thr183 and Tyr185 phosphorylation sites of human SAPK/JNK | | Form: Liquid | | Formulation: In Dulbecco's PBS (without Mg2+ and Ca2+), 50% glycerol, 1 mg/ml BSA, pH 7.3. | | Preservative: ≤0.1% sodium azide | | Comments: Recognizes the ~49 kDa SAPK1 protein and the ~55 kDa SAPK2 protein phosphorylated at Thr183 and Tyr185. | | Ref.: Fanger, G.R., et al. 1999. Histol. Histopathol. 14, 887. Gabai, V.L., et al. 1998. FEBS Lett. 438, 1. |
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* Additional Product Information
| | C. elegans, chicken, Drosophila, human, mouse, rat | IB, IH
See Key |
Detection of human JNK1 and JNK2, phosphorylated on Tyr183 and Thr185, by immunoblotting. Samples: Whole cell lysate from HEK293 cells left untreated (lane 1) or treated with UV irradiation (lanes 2-6). Primary antibody: PhosphoDetect™ Anti-SAPK/JNK (pTyr183,pThr185) Rabbit pAb (Cat. No. 559309) (1:1000) pre-incubated without peptide (lanes 1,6) or with immunogen phosphopeptide (lane 2), a generic phosphothreonine-containing peptide (lane 3), a generic phosphotyrosine-containing peptide (lane 4), or non-phosphopeptide corresponding to the immunogen (lane 5). Detection: chemiluminescence.
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