| | BacVector®-3000 Transfection Kit |
|  | Cat. No. 70077 |
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| Convenient high-efficiency construction of recombinant baculoviruses | | | BacVector® Transfection Kits are designed for efficient and reliable construction of baculovirus recombinants starting with pBAC™, pTriEx™, or other compatible transfer plasmid DNA. The BacVector Triple Cut Virus DNA has been digested with a restriction enzyme that cuts within the essential gene ORF1629, upstream of the lacZ gene and polh promoter, and within the lacZ gene (Kitts 1993). BacVector Triple Cut Virus DNA thus has an extremely low non-recombinant background, and typically produces nearly 100% recombinant plaques. BacVector Transfection Kits also contain Insect GeneJuice® Transfection Reagent, Sf9 Insect Cells, BacPlaque™ Agarose, and a Transfection Control Plasmid, all of which have been qualified for optimal transfection performance. BacVector DNA Kits contain the appropriate Triple Cut Virus DNA and matched Insect GeneJuice® Transfection Reagent, and a Transfection Control Plasmid. | | | | 1. Kitts, P.A. and Possee, R.D. 1993 BioTechniques 14, 810. | | | | | |
Note- this product is not available in Japan Components:
| • | 2 × 0.6 µg | BacVector-3000 Triple Cut Virus DNA
| | • | 2 µg | Transfection Control Plasmid
| | • | 3 vials | Sf9 Cells
| | • | 2 × 3 g | BacPlaque™ Agarose
| | • | 2 × 50 µl | Insect GeneJuice® Transfection Reagent
| | • | 2 × 500 µl | X-Gluc Solution
| | • | 1.5 ml | Nuclease-free Water |
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| Need additional information about this product? Email our Technical Service department at: novatech@novagen.com |
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Merck Chemicals list price is displayed (pricing with local distributors may vary). NOTE: In Stock status is based on item availability worldwide.
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Merck Chemicals list price is displayed (pricing with local distributors may vary). NOTE: In Stock status is based on item availability worldwide.
| BacVector®-2000 Transfection Kit | 70030-3 | 1 kit | * | N/A | | BacVector®-1000 Transfection Kit | 70059-3 | 1 kit | * | N/A |
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