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 | ≤ -70°C |  | Dry ice |  | Multiple Toxicity Values, refer to MSDS | | Note: Store and Ship conditions may differ. | | See Key |
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| | Tuner™(DE3)pLacI Competent Cells |
|  | Cat. No. 70625 |
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| Hosts specifically designed for T7-driven protein expression with pETBlue and pTriEx vectors | | | Expression host strains for pETBlue™ and pTriEx™ vectors are lDE3 lysogens that also carry the compatible pLacI plasmid, encoding the lac repressor. The pLacI plasmid confers chloramphenicol resistance and provides enough lac repressor to inhibit transcription from the T7lac promoter in the absence of inducer. The NovaBlue host strain is recommended for initial cloning with pETBlue and pTriEx vectors due to its superior transformation efficiency and high yields of high-quality plasmid DNA.
Note that these strains are not recommended for use with pET, pACYCDuet™, pCDF, pRSF, pCOLADuet™ or pETcoco™ vectors. | | | | | |
Tuner™ strains are lacZY deletion mutants of BL21 and enable adjustable levels of protein expression throughout all cells in a culture. The lac permease (lacY) mutation allows uniform entry of IPTG into all cells in the population, which produces a concentration-dependent, homogeneous level of induction. By adjusting the concentration of IPTG, expression can be regulated from very low levels up to the robust, fully induced levels commonly associated with pET vectors. Lower-level expression may enhance the solubility and activity of difficult target proteins. In Tuner(DE3)pLacI, the rare tRNA genes are present on the same plasmid that carries the lac repressor gene.
DE3 indicates that the host is a lysogen of lDE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter. Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG. Genotype: F– ompT hsdSB (rB– mB–) gal dcm lacY1(DE3) pLacI (CamR) Guaranteed Efficiency: Guaranteed efficiency: > 2 x 106 cfu/mg Components:
| 0.4 ml | 1 ml | Component
| | • | 2 × 0.2 ml | 5 × 0.2 ml | Tuner(DE3)pLacI Competent Cells
| | • | 2 × 2 ml | 4 × 2 ml | SOC Medium
| | • | 2 ng | 2 ng | Test Plasmid |
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| Need additional information about this product? Email our Technical Service department at: novatech@novagen.com |
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* Additional Product Information
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