| | Rosetta™(DE3)pLacI Competent Cells |
|  | Cat. No. 70920 |
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| Hosts specifically designed for T7-driven protein expression with pETBlue and pTriEx vectors | | | Expression host strains for pETBlue™ and pTriEx™ vectors are lDE3 lysogens that also carry the compatible pLacI plasmid, encoding the lac repressor. The pLacI plasmid confers chloramphenicol resistance and provides enough lac repressor to inhibit transcription from the T7lac promoter in the absence of inducer. The NovaBlue host strain is recommended for initial cloning with pETBlue and pTriEx vectors due to its superior transformation efficiency and high yields of high-quality plasmid DNA.
Note that these strains are not recommended for use with pET, pACYCDuet™, pCDF, pRSF, pCOLADuet™ or pETcoco™ vectors. | | | | | |
Rosetta™ 2 host strains are BL21 derivatives designed to enhance the expression of eukaryotic proteins that contain codons rarely used in E. coli. These strains supply tRNAs for 7 rare codones (AGA, AGG, AUA, CUA, GGA, CCC, and CGG) on a compatible chloramphenicol-resistant plasmid. The tRNA genes are driven by their native promoters. In Rosetta(DE3)pLacI, the rare tRNA genes are present on the same plasmid that carries the lac repressor gene.
DE3 indicates that the host is a lysogen of lDE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter. Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG. Genotype: F– ompT hsdSB (rB– mB–) gal dcm (DE3) pLacIRARE (CamR) Components:
| 0.4 ml | 1 ml | Component
| | • | 2 × 0.2 ml | 5 × 0.2 ml | Rosetta(DE3)pLacI Competent Cells
| | • | 2 × 2 ml | 4 × 2 ml | SOC Medium
| | • | 2 ng | 2 ng | Test Plasmid |
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| Need additional information about this product? Email our Technical Service department at: novatech@novagen.com |
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Merck Chemicals list price is displayed (pricing with local distributors may vary). NOTE: In Stock status is based on item availability worldwide.
* Additional Product Information
Merck Chemicals list price is displayed (pricing with local distributors may vary). NOTE: In Stock status is based on item availability worldwide.
| Origami™ B(DE3)pLacI Competent Cells | 70838-3 | 0.4 ml | Y | N/A* | | Origami™ B(DE3)pLacI Competent Cells | 70838-4 | 1 ml | Y | N/A* | | RosettaBlue™(DE3)pLacI Competent Cells | 71060-3 | 0.4 ml | Y | N/A* | | RosettaBlue™(DE3)pLacI Competent Cells | 71060-4 | 1 ml | Y | N/A* |
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* Additional Product Information
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