The pET-51b(+) vector is designed for cloning and high-level expression of target proteins fused with the 8 aa Strep•Tag ® II coding sequence that is cleavable with enterokinase (Ek) protease. The plasmid contains a strong T7lac promoter, an optimized RBS, the coding sequence for the Ek protease cleavage site (AspAspAspAspLys), and a multiple cloning site that contains restriction enzyme sites found in many other Novagen expression vectors to facilitate insert transfer. An optional C-terminal His•Tag ® coding sequence with 10 histidines is compatible with purification, detection, and quantification.

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