The pET-52b(+) vector is designed for cloning and high-level expression of target proteins fused with the 8 aa Strep•Tag^® II coding sequence that is cleavable with human rhinovirus (HRV) 3C protease. The plasmid contains a strong T7lac promoter, an optimized RBS, the coding sequence for the HRV 3C protease cleavage site (LysGluValLysPheGln↓GlyPro), and a multiple cloning site that contains restriction enzyme sites found in many other Novagen expression vectors to facilitate insert transfer. It also encodes an optional C-terminal thrombin protease cleavage site and His•Tag^® coding sequence with 10 histidines. The His•Tag fusion is compatible with purification, detection, and quantification.

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