Mass Spectrometry Results: All-in-One Trypsin Digestion Kit. BSA was digested using the ProteoExtract® All-in-One Trypsin Digestion Kit and the results were analyzed by LC-MS/MS. The mass spectrometer was operated in the positive ion mode, peak width of less than 0.6 µ, m/z range of 100–3000. The peptides were fragmented using auto-MS/MS, active exclusion, and two precursor ions. Protein identifications were made using the Matrix Science Mascot algorithm.
Mass Spectrometry Results: All-in-One Trypsin Digestion Kit/Arg C-like. BSA was digested using the ProteoExtract® All-in-One Trypsin Digestion Kit in combination with the Arg C-like modification and the results were analyzed by LC-MS/MS. The mass spectrometer was operated in the positive ion mode, peak width of less than 0.6 µ, m/z range of 100–3000. The peptides were fragmented using auto-MS/MS, active exclusion, and two precursor ions. Protein identifications were made using the Matrix Science Mascot algorithm.
Mass Spectrometry Results: All-in-One Trypsin Digestion Kit/Lys C-like. BSA was digested using the ProteoExtract® All-in-One Trypsin Digestion Kit in combination with the Lys C-like modification and the results were analyzed by LC-MS/MS. The mass spectrometer was operated in the positive ion mode, peak width of less than 0.6 µ, m/z range of 100–3000. The peptides were fragmented using auto-MS/MS, active exclusion, and two precursor ions. Protein identifications were made using the Matrix Science Mascot algorithm.
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