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 | 2 to 8°C |  | Ambient |  | Hazardous |  | Multiple Toxicity Values, refer to MSDS | | Note: Store and Ship conditions may differ. | | See Key |
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| | ProteoExtract® Tissue Dissociation Buffer Kit |
|  | Cat. No. 539720 |
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| All Categories » Calbiochem » Kits and Tools » Protein Extraction and Sample Preparation » Protein Extraction and Fractionation |
| Form: 10 Dissociation reactions, 1 g each | | Sample Type: Fresh tissue samples from eukaryotic organisms | | Kit Contents: 10X Tissue Dissociation Buffer, Protease Inhibitor Cocktail, Tissue Dissociation Buffer Additive and a user protocol. | | Comments: The ProteoExtract® Tissue Dissociation Buffer Kit is a tool for isolation of viable cells from fresh tissue. The isolated cells can then be used for total, subcellular, membrane, or other form of proteome extractions. This kit serves as an accessory product for the following kits: ProteoExtract® Subcellular Proteome Extraction Kit (Cat. No.: 539790), ProteoExtract® Subcellular Proteome Extraction Kit, Mini (Cat. No.: 539791), ProteoExtract® Native Membrane Protein Extraction Kit (Cat. No.: 444810). |
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Collagenases Used With Various Tissues.
Western Blot Analysis of Fractionated Proteins Following Tissue Dissociation. Tissue dissociation of rat pancreatic tissue and human pancreatic tissue was carried out according to the protocol outlined above. 1.3 x 107 dissociated cells were extracted using the ProteoExtract® Subcellular Proteome Extraction Kit protocol (Cat. No. 539790), incorporating the protocol changes as outlined above. Proteins from the cytosolic fraction, the membrane fraction, the nuclear fraction, and the cytoskeletal fraction were separated by SDS-PAGE, transferred to PVDF membrane, and probed with antibodies to the indicated markers.
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