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 | 2 to 8°C |  | Blue ice |  | Hazardous |  | Multiple Toxicity Values, refer to MSDS | | Note: Store and Ship conditions may differ. | | See Key |
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| | ProteoExtract® Phosphopeptide Enrichment TiO2 Kit |
|  | Cat. No. 539722 |
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| All Categories » Calbiochem » Kits and Tools » Protein Extraction and Sample Preparation » Protein Extraction and Fractionation |
| Format: Phosphopeptide enrichment | | Form: 100 Isolations | | Detection Method: Mass Spectrometry | | Kit Contents: TiO2 Phosphobind Resin, 10X TiO2 Phosphobind Buffer, Wash Buffer 1, Wash Buffer 2, TiO2 Elution Buffer, Dihydroxybenzoic Acid, and a user protocol. | | Comments: The ProteoExtract® Phosphopeptide TiO2 Enrichment Kit is a useful tool for enrichment of phosphorylated peptides from complex samples by a highly selective Titanium oxide solid phase. It enhances the ability to routinely identify and characterize large numbers of phosphorylated species within complex protein mixtures taking advantage of a novel titanium dioxide material. Captured and eluted phosphopeptide fractions are ready for MALDI- and ESI- mass spectrometry analysis. |
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Phosphopeptide Enrichment. Titanium dioxide allows for selective and sensitive phosphopeptide enrichment with low background from complex mixtures. A complex peptide mixture derived from a tryptic digest of porcine liver extract was spiked with a-casein and two synthetic phosphopeptides (see below for concentrations). The samples were subsequently processed using the ProteoExtract® Phosphopeptide Enrichment TiO2 Kit as outlined in the Detailed Protocol. Mass spectrometry analysis was performed using ESI-LC/MS equipment operated in positive mode. (A) Unprocessed sample (B) Phosphopeptides recovered using the ProteoExtract® Phosphopeptide Enrichment TiO2 Kit. Spiked Phosphopeptides: 1. 50 nM Angiotensin ~6 pmol/125 µl; ~7 ng/125 µl 2. 67 nM Calcineurin Substrate ~8 pmol/125 µl; ~18 ng/125 µl 3. 140 nM a-Casein ~18 pmol/125 µl; ~400 ng/125 µl
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