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 | 2 to 8°C |  | Blue ice |  | Hazardous |  | Multiple Toxicity Values, refer to MSDS | | Note: Store and Ship conditions may differ. | | See Key |
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| | ProteoExtract® Phosphopeptide Enrichment SCIMAC Kit |
|  | Cat. No. 539723 |
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| All Categories » Calbiochem » Kits and Tools » Protein Extraction and Sample Preparation » Protein Extraction and Fractionation |
| Format: Phosphopeptide enrichment | | Form: 100 Isolations | | Detection Method: Mass Spectrometry | | Kit Contents: 10X Phosphobind Buffer, Wash Buffer 1, Wash Buffer 2, Elution Buffer, MagPrep® Phosphobind Particles, SCX Resin, SCX Elute Buffer, and a user protocol. | | Comments: The ProteoExtract® Phosphopeptide Enrichment SCIMAC Kit is a useful tool for enrichment of phosphorylated peptides from complex samples by combination of strong cation exchange chromatography (SCX) and immobilized metal ion affinity chromatography (IMAC). The identification of phosphorylation sites is routinely accomplished by mass spectrometry. Captured and eluted phosphopeptide fractions are ready for MALDI- and ESI- mass spectrometry analysis. |
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Phosphopeptide Enrichment. The combination of SCX and IMAC exhibits high selectivity for phosphopeptides by reducing nonspecific binding of peptides from complex mixtures. A complex peptide mixture derived from a tryptic digest of porcine liver extract was spiked with a-casein and two synthetic phosphopeptides. The samples were subsequently processed using (A) an ironoxide-based IMAC material alone or (B) the combination of SCX with zirconium-based IMAC particles as outlined in the Detailed Protocol. Mass spectrometry analysis was performed using ESI-LC/MS equipment operated in positive mode.
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