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Cytochrome c -Mediated Apoptosis

Kit Resource
  ProteoExtract® Cytosol/Mitochondria Fractionation Kit
Cat. No. QIA88  
All Categories » Calbiochem » Kits and Tools » Protein Extraction and Sample Preparation » Protein Extraction and Fractionation

Format: Cell extraction
Form: 100 Extractions
Sample Type: Cells
Kit Contents: Mitochondria Extraction Buffer, Cytosol Extraction Buffer, Protease Inhibitor Cocktail, Reducing Reagent, and a user protocol.
Comments: This kit provides a unique combination of reagents useful for the isolation of a highly enriched mitochondrial fraction from the cytosol. The enriched fractions can be used to study factors of interest using Western blotting, ELISA, or other assays.
Note: 1 Test = reagents sufficient for processing 5 x 107 cells.
R: 23/24/25-32-34-36/37/38-41; S: 23-26-36/37/39-45

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Related Literature:

Caspases and other Apoptosis Related Tools Brochure
Tools and Tips for Analyzing Apoptosis

Material Safety Data Sheets:
QIA88: ProteoExtract® Cytosol/Mitochondria Fractionation Kit - English
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Selected Citations:
  1. Saquib A. Lakhani, et al. (2006) Caspases 3 and 7: Key Mediators of Mitochondrial Events of Apoptosis. Science 311, 847-851.
  2. Rupesh Chaturvedi, et al. (2004) Induction of Polyamine Oxidase 1 by Helicobacter pylori Causes Macrophage Apoptosis by Hydrogen Peroxide Release and Mitochondrial Membrane Depolarization. Journal of Biological Chemistry 279, 40161-40173.